CAPS2

Training period on Vibrio spp. detection (15 - 19 September 2014)

From 15 to 19 September 2014k, two representatives of Croatian Veterinary Institute of Split visited IZSAM laboratories to attend a training period on Vibrio spp. Detection.

The training involved Food Hygiene Unit of IZSAM, where they  could learn relevant elements of the molecular methods for Vibrio spp. detection and quality controls associated to a PCR protocol, the ISO regulating the approach for the 3 estimation of measurement uncertainty and  also gain more knowledge on Vibrio parahaemolyticus virulence genes detection.

After a general introduction in class room, the trainees moved in lab and they have been trained on

ISO 21872/2007 and multiplex PCR Vibrio spp. detection, focusing on sample preparation, quality controls, microbiology of food and animal feeding stuffs,  Vibrio spp. biochemical confirmation tests and results, DNA extraction from suspected, global approach for the estimation of measurement uncertainty and reproducibility standard deviation and expression of measurement uncertainty.

 IMGP0757 2

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